Quantitative relationship of Treponema denticola to severity of periodontal disease

We report the use of immunochemical assay methods with monoclonal antibodies for the detection and quantitation of the level of T. denticola in human clinical samples

L G Simonson; C H Goodman; J J Bial; H E Morton

2020

Scholarcy highlights

  • The Treponema denticola content of plaque was quantitatively estimated for samples taken from periodontitis patients as well as periodontally healthy subjects among two separate human populations
  • The sensitivity increased about fivefold, the specificity of monoclonal antibody TDII,IAA11 was as reported previously with an earlier indirect enzyme-linked immunosorbent assay procedure
  • The specific spirochete is a serotype of T. denticola
  • Listgarten and Levin found that only spirochetes appeared to be positively correlated with probing depth. We extend these findings by identifying and quantitating a specific component of the spirochete flora which increases with periodontal disease severity
  • The most probable reason for lower values in the military population is that the samples included both supragingival and subgingival plaque, whereas the university samples were only subgingival plaque. This had the effect of greatly diluting the relative T. denticola content, since spirochete populations are usually associated with subgingival, not supragingival, plaque
  • The two populations were studied separately and independently, some comparisons can be made to explain the much lower quantities of T. denticola antigen present in the military population versus the university population

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