Cloning and characterization of a second member of the mouse mdr gene family.

We have shown that one member, mdr1, has the capacity to convey multidrug resistance to drug-sensitive recipient cells in a gene transfer protocol

P Gros; M Raymond; J Bell; D Housman


Scholarcy highlights

  • The mammalian mdr gene family comprises a small number of closely related genes
  • The predicted amino acid sequence of this protein showed that it is a membrane glycoprotein highly homologous to mdrl, strongly suggesting that both genes originate from a common ancestor
  • Knowledge of the complete nucleotide sequence and predicted amino acid sequence of the mdr2 gene product will enable the preparation of gene-specific probes and antibodies necessary to study the functional role of this gene in multidrug resistance and normal physiological processes
  • A large body of experimental evidence indicates that the human, mouse, and hamster indr and P-glycoprotein gene families are composed of a small number of closely related genes
  • The multiplicity of the mdr gene family was first detected by unique cloned rndr probes in hybridization studies of genomic DNA from multidrug-resistant cell lines
  • We have previously reported the cloning of two families of DNA clones complementary to two indr genes from a cDNA library constructed with mRNA obtained from a drug-sensitive BALB/c mouse cell line
  • The strong nucleotide sequence homology between mouse mdri and indr2 genes indicates that a fulllength cDNA for either gene would not discriminate efficiently between mdrl and mdr2 transcripts in hybridization experiments

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