Detection of Yersinia pestis fraction 1 antigen with a fiber optic biosensor.

These results demonstrated that the use of the normalizing method in determining the F1 antigen concentration in these body fluids is valid

L K Cao

2020

Scholarcy highlights

  • Most immunoassay kits currently used in the field are singleuse tests which merely provide a positive or a negative response
  • The difficulties that exist both in providing excitation light and in obtaining a fluorescent signal have limited use of the evanescent wave sensing with clad optical fibers
  • The fibers were first coated with rabbit anti-plague immunoglobulin G and were incubated with 500 ng of F1
  • Three different antibodies directed toward the F1 antigen, two murine monoclonal antibodies and a polyclonal rabbit anti-plague IgG, were available to act either as the capture antibody or as the secondary labeled antibody
  • Previous experiments have established that antibodies immobilized onto the optical fiber surface retained the majority of their activity in storage for periods of up to 19 months
  • The fact that concentrations of F1 antigen in blood, serum, or plasma can be accurately derived from the standard curve generated by using F1 antigen in phosphate-buffered saline indicates that this method minimizes matrix effects
  • The fiber optic biosensor data correlated well with the actual concentrations, and no false-positive results were reported

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