The Escherichia coli argU10(Ts) Phenotype Is Caused by a Reduction in the Cellular Level of the argU tRNA for the Rare Codons AGA and AGG

We found that this mutation

Kensaku Sakamoto

2004

Scholarcy highlights

  • The E. coli argU10(Ts) mutation in the argU gene inhibits DNA replication, which stops cell growth at a high tempera
  • An in vivo analysis of aminoacylation levels showed that 30% of the argU10(Ts) tRNA molecules in the mutant cells are deacylated at 30°C, while most of the argU tRNA molecules in the wild-type cells are aminoacylated
  • The temperature-sensitive growth of SF151 can be complemented by the wild-type argU gene on a low-copy vector derived from the ColIb-P9 plasmid, whose copy number is 1.7
  • The argU10(Ts) mutation was isolated as a mutation that inhibits DNA synthesis at a high temperature and was originally designated dnaY10(Ts). This mutation occurs in the gene encoding the minor tRNAArg for the AGA and AGG codons, the argU tRNA, and confers pleiotropic effects at the permissive and nonpermissive temperatures, including inhibition of translation of AGA and AGG codons
  • The present study provided further evidence for this claim by showing that the arginine-accepting ability of the argU tRNA, another translational activity, is crucial for the complementation
  • We created and used SF151, an argU10(Ts) mutant with strict temperature sensitivity, in order to judge the complementation by the argU tRNA variants unambiguously
  • The in vivo amount of the argU10(Ts) tRNA in the arginylated form is only 5% of the amount of the argU tRNA in wild-type cells, the expression of the E coli genes essential for cell growth hardly seems to be impaired at this temperature

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