Hypersensitivity to Hg2+ and hyperbinding activity associated with cloned fragments of the mercurial resistance operon of plasmid NR1.

We report on the properties of cells harboring hybrid plasmids containing the vector plasmid ColEl plus restriction endonuclease EcoRI fragments, known from previous studies to come plasmid NR1 or with plasmid pRR35 (a mutant plasmid with Tn3 inserted into the merA gene of plasmid

H Nakahara

2020

Scholarcy highlights

  • The region of plasmid NR1 concerned with resistance to Hg2' and organomercurials consists of sequences found on restriction endonuclease fragments EcoRI-H and EcoRI-I
  • Escherichia coli strain KP245 met his trp thy lac gal was used as host for the hybrid plasmids, which consisted of the ColEl::TnA plasmid vector into which EcoRI fragments from plasmid NR1 were cloned
  • We report on the properties of cells harboring hybrid plasmids containing the vector plasmid ColEl plus restriction endonuclease EcoRI fragments, known from previous studies to come plasmid NR1 or with plasmid pRR35 (a mutant plasmid with Tn3 inserted into the merA gene of plasmid
  • The recombinant plasmids used in this study have recently been described; pRR130 is a recombinant ColEl::TnA plasmid with cloned EcoRI fragments H and I from plasmid NR1
  • The structural gene for mercuric reductase is apparently cleaved by restriction endonuclease EcoRI
  • Attempts to measure binding of the organomercurials directly showed that both sensitive and hypersensitive cells bound less than 1% of the organomercurials and showed no differences in this regard from the resistant strain

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