Inhibition of Selenium Metabolism in the Oral Pathogen Treponema denticola

In this report we provide evidence that the antimicrobial action of stannous salts and a gold drug, auranofin, against Treponema denticola is mediated through inhibition of the metabolism of selenium for synthesis of selenoproteins

Sarah Jackson-Rosario; William T. Self

2009

Scholarcy highlights

  • The biological use of selenium as a catalyst, incorporated into proteins as selenocysteine, is broad
  • In this report we provide evidence that the antimicrobial action of stannous salts and a gold drug, auranofin, against Treponema denticola is mediated through inhibition of the metabolism of selenium for synthesis of selenoproteins
  • We examine the possibility that stannous ions interfere with selenium metabolism in T. denticola
  • The antimicrobial property of the fluoride ion alone does not appear to be related to T. denticola‚Äôs dependence on selenoproteins for growth. These results suggest that an intersection exists between selenium metabolism and stannous salt toxicity
  • Despite the decrease in total protein, both stannous chloride and auranofin reduced selenoprotein synthesis compared to the level in the control. While this reduction was expected with auranofin, based on our recently published results from studying selenium metabolism in Clostridium difficile, the effect of stannous chloride provides additional evidence that stannous ions interfere with selenium metabolism in this organism
  • Given that selenium is required for the synthesis of glycine reductase and, acetyl phosphate for ATP synthesis, we proposed that that is the root of growth inhibition
  • We can determine whether stannous salts inhibit selenium uptake by T. denticola by following the assimilation of 75Se in whole cells

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