Quantifying Serum Antiplague Antibody with a Fiber-Optic Biosensor

Yersinia pestis, the etiologic agent of bubonic plague, continues to be endemic in many parts of the world

George P. Anderson

2019

Scholarcy highlights

  • Yersinia pestis, the etiologic agent of bubonic plague, continues to be endemic in many parts of the world
  • The fraction 1 antigen preparation used in this study was the same as that utilized in the hybridoma screening process. 6H3-immunoglobulin G was affinity purified on a 3-ml Avidchrom column, by eluting the bound antibody with 0.1 M sodium acetate plus 20% glycerol
  • The competitive assay with probes coated with F1 antigen failed to provide adequate concentration discrimination
  • While a large signal decrease occurred after exposure to 5 ␮g of 6H3-IgG/ml, the F1 antigen-coated probes required very high concentrations of antibody to block subsequent signal generation
  • A small amount of F1 antigen was bound to these probes
  • Of the various methods investigated, the competitive assay with probes coated with rabbit antiplague IgG was selected for an additional trial to quantify antiplague IgG in a series of samples supplied by USAMRIID
  • The samples were tested neat by competitive immunoassay and diluted and retested if necessary to obtain a signal ratio on a responsive portion of the standard curve

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