Sensitive assay, based on hydroxy fatty acids from lipopolysaccharide lipid A, for Gram-negative bacteria in sediments.

The present paper describes a method for the optimization of the assay of LPS from sediments, using a simpler extraction that is more sensitive and at the same time yields specific information on the gram-negative bacterial community structure

J H Parker

2020

Scholarcy highlights

  • Biochemical measures have provided insight into the biomass and community structure of sedimentary microbiota without the requirement of selection by growth or quantitative removal from the sediment grains
  • This study used the assay of the hydroxy fatty acids released from the lipid A of the lipopolysaccharide in sediments to provide an estimate of the gram-negative bacteria
  • The lipid-extracted residue was hydrolyzed in 1 N HCI, and the hydroxy fatty acids of the lipopolysaccharide were recovered in chloroform for analysis by gas-liquid chromatography
  • The present paper describes a method for the optimization of the assay of LPS from sediments, using a simpler extraction that is more sensitive and at the same time yields specific information on the gram-negative bacterial community structure
  • The phenol-water and the trichloroacetic acid extractions yielded 98 and 245% of the values reported for E. coli-mud mixtures by Saddler and Wardlaw
  • Muramic acid can be determined by the application of an additional acid hydrolysis of the residue extracted for the LPS-lipid A hydroxy fatty acids

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