The Tomato Rhizosphere, an Environment Rich in Nitrogen-Fixing Burkholderia Species with Capabilities of Interest for Agriculture and Bioremediation

The results revealed a high level of diversity of diazotrophic Burkholderia species, including B. unamae, B. xenovorans, B. tropica, and two other unknown species, one of them phylogenetically closely related to B. kururiensis

Jesús Caballero-Mellado; Janette Onofre-Lemus; Paulina Estrada-de los Santos; Lourdes Martínez-Aguilar


Scholarcy highlights

  • It is well known that hundreds of thousands of bacterial species remain to be discovered and cultured, representing a substantial reservoir of genetic diversity and great potential for biotechnological applications
  • Fixing species B. xenovorans was described on the basis of three isolates ; strain LB400T was isolated from polychlorinated biphenyl-contaminated soil in Moreau, NY, strain CAC-124 was isolated from the rhizosphere of a coffee plant cultivated in Veracruz, Mexico, and strain CCUG 28445 was recovered from a blood culture in Sweden
  • We found that the rhizosphere of tomato is a reservoir of different known and unknown diazotrophic Burkholderia species that are able to exhibit in vitro some activities involved in bioremediation, plant growth promotion, and biological control
  • A total of eight ARDRA profiles were identified from among the 54 diazotrophic Burkholderia isolates, and on this basis, only 25 representative N2-fixing isolates of each ARDRA genotype identified among isolates recovered from tomato plants cultivated on different farms were further analyzed
  • Two other unknown nitrogen-fixing Burkholderia species were cultured in the present study, one of them closely phylogenetically related to B. kururiensis and the other one to legume-nodulating strains and to B. silvatlantica
  • The lack of correlation between the largest orange haloes exhibited on chrome azurol S-Casamino Acids medium and the very small amounts of hydroxamate siderophores found in liquid cultures from B. xenovorans isolates associated with tomato plants suggests that other types of siderophores, different from hydroxamates, are produced by strains of these species

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