Complementary DNA Sequencing: Expressed Sequence Tags and Human Genome Project

Automated partial DNA sequencing was conducted on more than 600 randomly selected human brain complementary DNA clones to generate expressed sequence tags

Mark D. Adams; Jenny M. Kelley; Jeannine D. Gocayne; Mark Dubnick; Mihael H. Polymeropoulos; Hong Xiao; Carl R. Merril; Andrew Wu; Bjorn Olde; Ruben F. Moreno; Anthony R. Kerlavage; W. Richard McCombie; J. Craig Venter

2006

Scholarcy highlights

  • Automated partial DNA sequencing was conducted on more than 600 randomly selected human brain complementary DNA clones to generate expressed sequence tags
  • Forty-six ESTs were mapped to chromosomes after amplification by the polymerase chain reaction
  • This fast approach to complementary DNA characterization will facilitate the tagging of most human genes in a few years at a fraction of the cost of complete genomic sequencing, provide new genetic markers, and serve as a resource in diverse biological research fields

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