Expansion microscopy

CESM and MERRA show many similarities; we find that CESM requires less power to maintain its hydrological cycle than the reanalysis, due to the smaller amplitude of its moistening inefficiencies

F. Chen

2015

Scholarcy highlights

  • By covalently anchoring specific labels located within the specimen directly to the polymer network, labels spaced closer than the optical diffraction limit can be isotropically separated and optically resolved, a process we call expansion microscopy
  • We demonstrate ExM with apparent ~70-nanometer lateral resolution in both cultured cells and brain tissue, performing three-color superresolution imaging of ~107 cubic micrometers of the mouse hippocampus with a conventional confocal microscope
  • We labeled features traditionally used to characterize the performance of superresolution microscopes, including microtubules and clathrin coated pits, and imaged them with a superresolution structured illumination microscope pre-ExM, and a spinning disk confocal post-ExM
  • Coordinated replication and expression of the mitochondrial genome is critical for metabolically active cells during various stages of development. It is not known whether replication and transcription can occur simultaneously without interfering with each other and whether mitochondrial DNA copy number can be regulated by the transcription machinery.We found that interaction of human transcription elongation factor TEFM with mitochondrial RNA polymerase and nascent transcript prevents the generation of replication primers and increases transcription processivity and thereby serves as a molecular switch between replication and transcription, which appear to be mutually exclusive processes in mitochondria
  • We found that the termination efficiency of mitochondrial RNA polymerase was substantially lower at G5AG7-conserved sequence block II, which suggested an effect of G run length on quadruplex formation and underscored the importance of further studies of various polymorphisms in this region
  • The performance of expansion microscopy suggests that despite statistical fluctuations in polymer chain length at the molecular scale, at the nanoscale distances here examined these fluctuations average out, yielding isotropy

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