Dynamic Proteomics of Individual Cancer Cells in Response to a Drug

Why do seemingly identical cells respond differently to a drug? To address this, we studied the dynamics and variability of the protein response of human cancer cells to a chemotherapy drug, camptothecin

A. A. Cohen


Scholarcy highlights

  • We used two rounds of CD tagging with the red fluorescent protein mCherry to obtain a cell clone with red fluorescence in the cytoplasm and stronger red fluorescence in the nucleus
  • We introduced an enhanced yellow fluorescent protein into the red-tagged cells by an additional round of CD tagging, expanded the yellow-tagged cells into clones, and identified the yellow-tagged proteins
  • Dynamic changes as small as 20 to 30% in tagged protein intensity typically can be accurately detected by using the present assay in individual cells
  • This study provided a view of the response to a drug in space and time for about 1000 proteins in individual cells
  • We identified an RNA helicase and a DNA replication factor, whose dynamics varied widely between cells, in a way that corresponds to cell fate: They rise in cells that survive and decrease in cells that die
  • Understanding the human cell as a dynamical system will require viewing it on several levels, including mRNA and protein levels, modifications, and localizations, in individual living cells over time

Need more features? Save interactive summary cards to your Scholarcy Library.