Initiation of Mammalian Liver Development from Endoderm by Fibroblast Growth Factors

The signaling molecules that elicit embryonic induction of the liver from the mammalian gut endoderm or induction of other gut-derived organs are unknown

Joonil Jung; Minghua Zheng; Mitchell Goldfarb; Kenneth S. Zaret

2002

Scholarcy highlights

  • The signaling molecules that elicit embryonic induction of the liver from the mammalian gut endoderm or induction of other gut-derived organs are unknown
  • Close proximity of cardiac mesoderm, which expresses fibroblast growth factors 1, 2, and 8, causes the foregut endoderm to develop into the liver
  • Treatment of isolated foregut endoderm from mouse embryos with FGF1 or FGF2, but not FGF8, was sufficient to replace cardiac mesoderm as an inducer of the liver gene expression program, the latter being the first step of hepatogenesis
  • For FGF1 and FGF2 localization, sections were treated with 0.25% trypsin in phosphate-buffered saline for 5 min at room temperature
  • Mouse monoclonal antibodies against FGF1 were obtained from Sigma, and monoclonal antibody against FGF2, bFM-2, has been described [K
  • Mouse monoclonal antibody against FGF8 was obtained from R&D systems
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