A Mammalian Scaffold Complex That Selectively Mediates MAP Kinase Activation

The c-Jun NH2-terminal kinase group of mitogen-activated protein kinases is activated by the exposure of cells to multiple forms of stress

Alan J. Whitmarsh; Julie Cavanagh; Cathy Tournier; Jun Yasuda; Roger J. Davis

2002

Scholarcy highlights

  • The c-Jun NH2-terminal kinase group of mitogen-activated protein kinases is activated by the exposure of cells to multiple forms of stress
  • A putative scaffold protein was identified that interacts with multiple components of the JNK signaling pathway, including the mixed-lineage group of MAP kinase kinase kinases, the MAP kinase kinase MKK7, and the MAP kinase JNK
  • This scaffold protein selectively enhanced JNK activation by the MLK signaling pathway. These data establish that a mammalian scaffold protein can mediate activation of a MAP kinase signaling pathway
  • An alternatively spliced JIP-1 cDNA was isolated from a murine brain library
  • Further studies are required to determine whether IB1, like JIP-1 and JIP-1b, can act as a scaffold protein that binds components of the JNK MAP kinase signaling module
  • Bacterial expression plasmids for epitope-tagged MKK7 and MLK3 were constructed by inserting Flag-MKK7 and Flag-MLK3 into pRSETA
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