DETECTION OF GLUTATHIONE CONJUGATES DERIVED FROM 4-IPOMEANOL METABOLISM IN BILE OF RATS BY LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY

Earlier studies postulated that bioactivation of 4-ipomeanol by cytochrome P450 enzymes may occur through oxidation of its furan ring, following a mechanism similar to the bioactivation of other furan-containing compounds

Teresa M. Alvarez-Diez; Jiang Zheng

2004

Scholarcy highlights

  • Earlier studies postulated that bioactivation of 4-ipomeanol by cytochrome P450 enzymes may occur through oxidation of its furan ring, following a mechanism similar to the bioactivation of other furan-containing compounds
  • This would lead to the formation of furan epoxides and α,β-unsaturated di-aldehyde-reactive metabolites that can conjugate with glutathione
  • We analyzed by liquid chromatography-tandem mass spectrometry the bile of rats administered d0/d6 4-ipomeanol intravenously
  • Hexadeuterated 4-ipomeanol had all deuterium atoms incorporated on its aliphatic chain
  • Multiple reaction monitoring scans of bile for the mass transition: MH+/(MH - 129)+, which is characteristic of glutathione conjugates, detected four glutathione conjugates
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