Transcriptional regulation of aquaporins in accessions of Arabidopsis in response to drought stress

We have previously shown that major intrinsic proteins transcription in Arabidopsis thaliana is generally downregulated in leaves upon drought stress, apart from two members of the plasma membrane intrinsic protein subfamily, AtPIP1;4 and AtPIP2;5, which are upregulated

Erik Alexandersson

2009

Scholarcy highlights

  • In plants, aquaporin homologs, called major intrinsic proteins, form a large protein family, with 35 identified members in Arabidopsis, and 33 in maize and rice
  • With the help of the Arabidopsis thaliana trans-factor and cis-element prediction database, and other publicly available microarray data and bioinformatics tools, we show that several of the MIPs downregulated upon drought stress are strongly co-expressed, whereas AtPIP1;4, AtPIP2;5 and AtPIP2;6 do not co-express with this group
  • AtPIP1;4 and AtPIP2;5 were upregulated for all five accessions, whereas the remaining plasma membrane intrinsic protein genes, with the exception of AtPIP2;6, showed a general downregulation
  • The expression of AtPIP2;8 appears to be sevenfold higher in Lip-0 compared with Col-0, whereas differences in expression for the other PIP transcripts were within a twofold change, and no major differences in expression intensity between accessions can be seen for AtPIP1;4, AtPIP2;5 and AtPIP2;6
  • We show that the general pattern of drought regulation of PIPs is conserved among five accessions of Arabidopsis
  • Future investigations should further clarify the physiological role of the general drought stress expression pattern of AQPs in plants, as well as the relevance of the identified natural variation in PIP expression among accessions
  • Ratios of mRNA levels of drought-stressed and control plants for the 13 plasma membrane intrinsic protein transcripts in five different accessions of Arabidopsis

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