Differential expression of two glutathione S-transferases identified from the American dog tick, Dermacentor variabilis

Reciprocal signalling and gene expression play a cardinal role during pathogen–host molecular interactions and are prerequisite to the maintenance of balanced homeostasis

S. M. Dreher-Lesnick; A. Mulenga; J. A. Simser; A. F. Azad

2006

Scholarcy highlights

  • Reciprocal signalling and gene expression play a cardinal role during pathogen–host molecular interactions and are prerequisite to the maintenance of balanced homeostasis
  • GSTs are well known to play an important part in cellular stress responses in the host
  • We have cloned two full-length GSTs from D. variabilis. Comparison of these two DvGST molecules with those of other species indicate that DvGST1 is related to the mammalian class theta and insect class delta GSTs, while DvGST2 does not seem to fall in the same family
  • Northern blotting analyses revealed differential expression patterns, where DvGST1 and DvGST2 transcripts are found in the tick gut, with DvGST2 transcripts present in the ovaries
  • Challenge of fed adult ticks with Escherichia coli injection showed decreased transcript amounts compared with ticks injected with phosphate-buffered saline and naïve ticks

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