Comparison of the effect of a mitochondrial uncoupler, 2,4-dinitrophenol and adrenaline on oxygen radical production in the isolated perfused rat liver

Using the isolated perfused rat liver, we examined the effect of stimulation of mitochondrial respiration by 2,4-dinitrophenol and adrenaline on reactive oxygen species production, liver damage and lipid peroxidation

M. OKUDA; H.-C. LEE; C. KUMAR; B. CHANCE

2008

Scholarcy highlights

  • Using the isolated perfused rat liver, we examined the effect of stimulation of mitochondrial respiration by 2,4-dinitrophenol and adrenaline on reactive oxygen species production, liver damage and lipid peroxidation
  • ROS production was monitored by luminol- and lucigenin-enhanced chemiluminescence and oxygen uptake was measured simultaneously
  • Liver damage and lipid peroxidation were evaluated by measuring hepatic lactate dehydrogenase and thiobarbituric acid reacting substances release
  • On termination of 2,4-DNP infusion, there was a sharp increase in lucigenin-enhanced chemiluminescence, which declined slowly, but luminol-enhanced chemiluminescence did not change prominently
  • LDH and TBARS release were not affected significantly by adrenaline infusion. These results indicate that uncoupling of oxidative phosphorylation decreases ROS production and restoration of oxidative phosphorylation enhances ROS production and liver damage
  • Xanthine oxidase is unlikely to contribute to enhanced reactive oxygen species production after termination of 2,4-DNP but has some protective effect during uncoupling

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