RECOVERY OF ESCHERICHIA COLI O157:H7 FROM FIBER OPTIC WAVEGUIDES USED FOR RAPID BIOSENSOR DETECTION

Consumption of ground beef has been implicated in the majority of outbreaks of disease from Escherichia coli O157:H7

MARIANNE F. KRAMER

2007

Scholarcy highlights

  • Consumption of ground beef has been implicated in the majority of outbreaks of disease from Escherichia coli O157:H7
  • Evanescent wave, fiber optic biosensors are an innovative, cutting‐edge technology with the potential to meet such a need. Food pathogens such as E. coli O157:H7 can be detected in minutes using the biosensor assay rather than days using conventional methods
  • After a positive identification on the biosensor, the waveguide was added to modified LB medium supplemented with 10 μg/mL acriflavin and incubated at 42C for a minimum of 4 h followed by selection on Rainbow O157 agar incubated 16 to 18 h at 37C
  • It was possible to recover E. coli O157:H7 colonies in E. coli O157:H7‐seeded PBS or ground beef when 1 mL samples containing at least 2.0 × 103 CFU of E. coli O157:H7 had been injected into the biosensor
  • The identification of isolated colonies was quickly confirmed using slide agglutination with the appropriate antiserum. This method significantly improved the speed of the confirmation of the presence of the pathogen and of the isolation of the pathogen, from 4 days using conventional methods to one day using the biosensor assay
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