A nondestructive, rapid, reliable and inexpensive method to sample, store and extract high-quality DNA from fish body mucus and buccal cells

A rapid, nondestructive, reproducible and cheap DNA extraction method from body mucus and buccal cells of northern pike and brown trout is described

LUCENTINI LIVIA; PALOMBA ANTONELLA; LANCIONI HOVIRAG; NATALI MAURO; FAUSTO PANARA

2006

Scholarcy highlights

  • A rapid, nondestructive, reproducible and cheap DNA extraction method from body mucus and buccal cells of northern pike and brown trout is described
  • Buccal cells and body mucus were sampled on FTA Cards; the captured DNA was used directly for microsatellite and polymerase chain reactionrestriction fragment length polymorphism analyses
  • The genotyping error rate for microsatellite ranged from 1.9% to 3.3% for the northern pike and brown trout, respectively
  • This methodology, using for the first time these materials as a fish DNA source, combines speed of sampling and processing, with a twofold to a threefold time and costs saving

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