Kinetics of the two-step hydrolysis of triacylglycerol by pancreatic lipases

We have considered lipolysis to be a

Athanasios Lykidis

2010

Scholarcy highlights

  • Pancreatic lipases catalyze the hydrolysis of triacylglycerol in a sequential manner
  • Pancreatic lipases catalyze the hydrolysis of triacylglycerol producing 1,2-diacyIglycerol, 2-monoacylglycerol, and fatty acids
  • We have investigated the effect of bile salt and colipase on the rates of triacylglycerol and diacylglycerol hydrolysis
  • We purified Human pancreatic lipase according to an established procedure for comparison to Rabbit pancreatic lipase
  • Our results indicate that the kinetic equations of sequential reactions provide a satisfactory description of the whole process of lipolysis
  • The main findings of the present study are summarized as follows : RPL hydrolyzes dioleoylglycerol faster than trioleoylglycerol; deoxycholate enhances dioleoylglycerol hydrolysis preferentially ; colipase leads to a similar increase in the rate of both reactions at high deoxycholate concentration in contrast to a slight selectivity toward trioleoylglycerol at low deoxycholate concentration ;(d) upon storage the enzyme suffers a greater loss of activity toward dioleoylglycerol as compared to trioleoylglycerol ; kinetic properties and stability patterns appear to be very similar between RPL and HPL
  • The main findings of the present study are summarized as follows : Rabbit pancreatic lipase hydrolyzes dioleoylglycerol faster than trioleoylglycerol; deoxycholate enhances dioleoylglycerol hydrolysis preferentially ; colipase leads to a similar increase in the rate of both reactions at high deoxycholate concentration in contrast to a slight selectivity toward trioleoylglycerol at low deoxycholate concentration ;(d) upon storage the enzyme suffers a greater loss of activity toward dioleoylglycerol as compared to trioleoylglycerol ; kinetic properties and stability patterns appear to be very similar between RPL and Human pancreatic lipase

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