Effect ofDionaea muscipulaextract and plumbagin on maceration of potato tissue byPectobacterium atrosepticum

We examined the response mechanism of Pectobacterium atrosepticum cells to Dionaea extract and plumbagin and compared it with the effect of a bactericidal peptide – CAMEL

A. Szpitter; M. Narajczyk; M. Maciag-Dorszynska; G. Wegrzyn; E. Lojkowska; A. Krolicka

2014

Scholarcy highlights

  • Pectobacterium atrosepticum is a plant pathogen that causes major crop losses
  • We examined the response mechanism of Pba cells to Dionaea extract and plumbagin and compared it with the effect of a bactericidal peptide – CAMEL
  • While the addition of the Dionaea extract and plumbagin caused a dramatic reduction in RNA and protein synthesis in Pba1043, it did not result in cellular damage
  • Plumbagin added to potato tubers inoculated with a field isolate Pba5A/1/2005 increased extracellular pectate lyase activity and reduced tissue maceration but did not affect bacterial counts per gram of tissue
  • These results show that plumbagin in the presence of compounds from potato tuber stimulates Pel production/secretion in Pba cells and increases the expression of the acrA gene
  • This may be the molecular basis for the less pronounced effects of Dionaea extract on Pectobacterium atrosepticum in planta relative to those observed in vitro

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