Immunomorphological and Ultrastructural Characterization of Langerhans Cells and a Novel, Inflammatory Dendritic Epidermal Cell (IDEC) Population in Lesional Skin of Atopic Eczema

We investigated epidermal cell suspensions prepared from lesional and nonlesional atopic eczen1.a skin, other inflammatory skin conditions, and normal human skin for high-affinity IgE receptor expression on dendritic CDla cells by quantitative flow cytometric analysis

Andreas Wollenberg

2004

Scholarcy highlights

  • ' Department of Dermato logy, Ludwig-Maximi li ans-Uni vc rsity of Muni ch, Munich, Germany; and i"Labora toire d'HistocompatibiJi tc, Etab lissc mcnt de Transfusion Sa nguine, Strasbourg, France
  • We investigated epidermal cell suspensions prepared from lesional and nonlesional atopic eczen1.a skin, other inflammatory skin conditions, and normal human skin for high-affinity IgE receptor expression on dendritic CDla cells by quantitative flow cytometric analysis
  • The highest Fce:RI expression was found on the second CDla population in lesional atopic eczema skin
  • A distinct population of CDla inflammatory dendritic epidermal cells different from classical Langerhans cells appears in the epidermis of lesional skin and is subjected to specific signals leading to the upregulation ofFce:RI in atopic eczema skin
  • G reat in terest in AE, in partic ul ar, ha s evo lved siL1 ce t h e presen ce of IgE m o lecul es has been re ported o n CD l a-positive Langerhans ce ll s in t hi s di sease, a nd we a nd othe rs hav e demonstrated the presence of th e hi g h-alti nity receptor for IgE, FCETU, o n t h ese cell s
  • Two Cellular Components in the Epidermal CDla/FcER! Population in Atopic Eczema Since it has been postulated that so m e h eterogen e ity in t h e Langerhans cell population may b e prese nt in AE in phe n otyp e and IgE bind in g, we first performed d etailed co ntou r plot ana lysis of th e FCERII CD l a ce ll s in norm a l sk in and AE, and extende d th e studies b y studying CD 1b, CD23, CD36, and HLA-DR expressio n
  • The observation ofFc£R.lIIC D 23 expression 0 11 both COla ceU populations in les io nal AE and pso ri as is provid es an ill silll correlation w ith the repo rted indu c tion of thi s recepto r on normal Langerhans cell s by in te d eukin-4 and interfe ron--y

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