Disease resistance conferred by expression of a gene encoding H2O2-generating glucose oxidase in transgenic potato plants.

To determine whether H2O2 was required for bacterial resistance, we investigated the effect of catalase on disease progression in transgenic tubers

G Wu

2002

Scholarcy highlights

  • Plants ward off pathogen infections by eliciting an array of defense mechanisms, including reinforcement of the cell wall, synthesis of phytoalexins and oxidation of phenolic compounds, activation of defense-related genes, and localization of cell death or the hypersensitive response
  • Extracts of potato leaf protein were subjected to SDS-PAGE and immunodetected using the antibody raised against A. niger glucose oxidase. GO detected in total leaf proteins of several transgenic potato lines
  • We showed that expression of a gene encoding H202
  • The results demonstrate that active oxygen species production can be manipulated in the plant through expression of a transgene and be linked to enhanced resistance of the plants to pathologically diverse pathogens
  • GO has previously been found to be the major antimicrobial metabolite of the fungus Talaromyces flavus used in the biological control of soil-borne pathogens, presumably through generation of H202
  • The effective control of both the bacterial soft rot disease and the funga1 late blight disease occurred by virtue of elevated levels of H202.That H202elevation accounts for the increased disease resistance shown by the GO transgenic plants is confirmed by our observation that, in the presence of catalase, resistance to soft rot could not be sustained
  • Bacterial titer on potato tuber discs was determined by suspending the macerated tissue in sterile water and plating a series of dilutions of the suspension on Luria-Bertani agar medium

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