TDIF Peptide Signaling Regulates Vascular Stem Cell Proliferation via theWOX4Homeobox Gene inArabidopsis

We report that a WUSCHEL-related HOMEOBOX gene, WOX4, is a key target of the TDIF signaling pathway

Yuki Hirakawa; Yuki Kondo; Hiroo Fukuda

2010

Scholarcy highlights

  • Meristems are plants’ stem cell tissues essential for lifelong growth and development
  • Arabidopsis seedlings were grown with 5 mM TDIF or P9A for 7 d, and the transcript levels of the seven WUSCHEL-related HOMEOBOX genes were measured using quantitative RT-PCR
  • These results suggest that WOX4 is the main WOX gene affected by the TDIF signal
  • TDIF did not cause the fragmentation of xylem strands in tdr-1 or tdr-1 wox4-1 double mutants. These results indicate that WOX4 does not contribute to the suppression of the xylem differentiation caused by TDIF-TDR signaling
  • We revealed that the phloem-originated secretory peptide TDIF/CLE41 acts as one such positional cue for stem cell maintenance in the vascular meristem
  • We showed WOX4 to be a downstream component of this signaling pathway, which seems to regulate proliferation but not xylem differentiation in the vascular stem cells, showing independency of the two functions for this stem cell maintenance signal
  • This fact supports the idea that the TDIF-TDR-WOX4 signaling pathway functions generally in secondary growth of the vascular meristem

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