Two Medicago truncatula Half-ABC Transporters Are Essential for Arbuscule Development in Arbuscular Mycorrhizal Symbiosis

We report a Medicago truncatula mutant, stunted arbuscule, in which arbuscule development is impaired and arbuscular mycorrhizal symbiosis fails

Quan Zhang; Laura A. Blaylock; Maria J. Harrison

2010

Scholarcy highlights

  • The majority of the vascular flowering plants, including most crop species of agronomic significance, are able to develop symbiotic associations with arbuscular mycorrhizal fungi
  • To identify AM symbiosis mutants, M2 seedlings from a M. truncatula ethyl methanesulfonate mutant population were inoculated with Glomus versiforme
  • Analyses of STR and STR2 promoter-GUS fusions indicated that expression levels in the vascular tissue were lower than the colonized cortical cells, and it is possible that YFPN-STR/YFPCSTR2 proteins are present at levels below detection
  • The AM symbiosis occurs broadly in the angiosperms and in the Arum-type AM symbioses that occur in most herbaceous plants, the arbuscules mediate mineral nutrient transfer to the plant and are critical for the symbiosis
  • Arbuscule development is a complex process that involves the terminal differentiation of the fungal hypha and concomitantly a transient cellular reorganization and transcriptional reprogramming of the root cortical cells
  • STR and its partner, STR2, which we identified by its relatedness and identical phylogenetic profile, are half-transporters of the ABCG subfamily
  • Histochemical Staining for GUS Activity in M. truncatula Roots Expressing a STR2 Promoter-UidA Fusion Construct

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