Duplication of Symbiotic Lysin Motif Receptors Predates the Evolution of Nitrogen-Fixing Nodule Symbiosis

We identified four LysMtype receptors controlling nodulation in P. andersonii: PanLYK1, PanLYK3, PanNFP1, and PanNFP2

Luuk Rutten; Kana Miyata; Yuda Purwana Roswanjaya; Rik Huisman; Fengjiao Bu; Marijke Hartog; Sidney Linders; Robin van Velzen; Arjan van Zeijl; Ton Bisseling; Wouter Kohlen; Rene Geurts

2020

Scholarcy highlights

  • Rhizobium nitrogen-fixing nodule symbiosis occurs in two taxonomic lineages: legumes and the genus Parasponia
  • We identified 16 P. andersonii genes encoding putative Lys motif-type receptors that grouped in all except one known orthogroups
  • Our analysis revealed that PanLYK1 and PanLYK3 originated from an ancient duplication, dividing this orthogroup into two subgroups that we named LYK-Ia and LYK-Ib
  • Transcomplementation studies in a L. japonicus LCO receptor double mutant, and CRISPRCas9 mutagenesis in P. andersonii, we identified four LysM-type receptors that function in LCO-driven nodulation in a nonlegume
  • As the Parasponia spp. and legume lineages diverged early in the nitrogen-fixing clade, the use of orthologous genes for rhizobium LCO perception supports the hypothesis of a shared evolutionary origin of LCO-driven nodulation
  • This makes it most probable that the capability of these receptors to perceive LCOs predates the evolution of the nitrogen-fixing nodulation trait
  • By using P. andersonii as a comparative system to legumes, we revealed two ancestral duplications of Lys motif-type LCO receptors that predate, and coincide with, the evolution of nitrogen-fixing nodules

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