We discovered that miR396 is regulated by SLENDER RICE1 in controlling cell proliferation
2020
SLENDER RICE1 and Oryza sativa INDETERMINATE DOMAIN2 Regulating OsmiR396 Are Involved in Stem Elongation
Key concepts
Scholarcy highlights
- GAs play key roles in controlling cell proliferation through the GIBBERELLIN INSENSITIVE DWARF1/DELLA-mediated pathway
- Coordination of SLENDER RICE1, OsIDD2, OsmiR396, and OsGRFs Is Critical for GA-Mediated Cell Proliferation
- The transgenic lines could produce tillers, flowers, and seeds similar to the wild type, implying that the roles of miR396/OsGRFs are mainly involved in controlling the growth rate rather than cell differentiation
- The micro difference of cell sizes in stem and suspension cells between wild-type and miR396OE plants indicated that the suppressed cell proliferation is likely the main cause for the dwarfism of the transgenic plants
- The observation that the mRNA levels of GA-induced cyclin Oryza sativa1 and cycOs2 were lower and could not be restored by applied GA3 in miR396OE plants implied that these cell cycle-related genes act downstream of miR396/OsGRFs and that their response to GA is mediated by miR396/OsGRFs
- From the observations that both stem length and the RNA level of miR396 could be regulated but OsIDD2 could not at protein/mRNA levels by applying GA3 in the OsIDD2OE lines and the wild type, we proposed that OsIDD2, promoting the expression of miR396, requires the coactivator of SLR1
- To generate transgenic rice overexpressing OsmiR396 and OsIDD2, the two genes were cloned and constructed into an expression vector of pCAMBIA1301, which has a constitutive ubiquitin promoter, Ubi1
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