SLENDER RICE1 and Oryza sativa INDETERMINATE DOMAIN2 Regulating OsmiR396 Are Involved in Stem Elongation

We discovered that miR396 is regulated by SLENDER RICE1 in controlling cell proliferation

Yuzhu Lu; Zhen Feng; Yunlong Meng; Liying Bian; Hong Xie; Kirankumar S. Mysore; Jiansheng Liang

2020

Scholarcy highlights

  • GAs play key roles in controlling cell proliferation through the GIBBERELLIN INSENSITIVE DWARF1/DELLA-mediated pathway
  • Coordination of SLENDER RICE1, OsIDD2, OsmiR396, and OsGRFs Is Critical for GA-Mediated Cell Proliferation
  • The transgenic lines could produce tillers, flowers, and seeds similar to the wild type, implying that the roles of miR396/OsGRFs are mainly involved in controlling the growth rate rather than cell differentiation
  • The micro difference of cell sizes in stem and suspension cells between wild-type and miR396OE plants indicated that the suppressed cell proliferation is likely the main cause for the dwarfism of the transgenic plants
  • The observation that the mRNA levels of GA-induced cyclin Oryza sativa1 and cycOs2 were lower and could not be restored by applied GA3 in miR396OE plants implied that these cell cycle-related genes act downstream of miR396/OsGRFs and that their response to GA is mediated by miR396/OsGRFs
  • From the observations that both stem length and the RNA level of miR396 could be regulated but OsIDD2 could not at protein/mRNA levels by applying GA3 in the OsIDD2OE lines and the wild type, we proposed that OsIDD2, promoting the expression of miR396, requires the coactivator of SLR1
  • To generate transgenic rice overexpressing OsmiR396 and OsIDD2, the two genes were cloned and constructed into an expression vector of pCAMBIA1301, which has a constitutive ubiquitin promoter, Ubi1

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