The Effects of Mastoparan on the Carrot Cell Plasma Membrane Polyphosphoinositide Phospholipase C

We demonstrate that mastoparan and the active analog Mas-7 activate the carrot plasma membrane PIP-/PIP,-PLC only if added to intact cells

M. H. Cho

2002

Scholarcy highlights

  • Ulate cytoskeletal structure cells were treated with 10 or 25 p~ wasp venom peptide mastoparan or the active analog Mas-7 there was a rapid loss of more than 70% ofphosphatidylinositol-4-monophosphate andinositol-l,4-P2 andinositol-l,4,5-P, respectively
  • When Mas-7 was added to isolated plasma membranes from control cells, it had no effect on PIP-PLC activity at low concentrations and inhibited PIP-PLC at concentrations greater than 10 p ~ In. addition, guanosine-5’-0(3-thiotriphosphate) had no effect on the PIP-PLC activity when added t o plasma membranes isolated from either the Mas-7-treatedor control cells
  • We have demonstrated that in vivo treatment of carrot cells with 10 and 25 FM mastoparan and Mas-7 increases PIP- and PIE',PLC
  • TP, recovered from whole cells, the PLC activity in membranes isolated from cells treatecl with 25 p~ Mas-17 was higher than that from the water c-ontrols
  • Our data indicate that activation of PLC in the carrot cells did not involve direct interaction of mastoparan with PIP or integral plasma membrane proteins in that, when Mas-7 was added to isolated plasma membrane, PLC activity did not increase
  • Plant Physiol 9 4 1820-1829 Chen Q, Boss WF Neomycin inhibits the PIP and PIP, stimulation of plasma membrane ATPase activity

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