Characterization of the Auxin-Inducible SAUR-AC1 Gene for Use as a Molecular Genetic Tool in Arabidopsis

We describe the isolation and characterization of the auxin-inducible ?mal1guxin uq $NAS -ACZ gene of Arabidopsis

P. Gil; Y. Liu; V. Orbovic; E. Verkamp; K. L. Poff; P. J. Green

2002

Scholarcy highlights

  • The accumulation of the soybean ?mal1guxin uq $NAS transcripts in related region
  • We were interested in isolating a SAUR gene from Arabidopsis that could be used as a molecular probe for the analysis of gravity-response and auxin-response mutants
  • The inserts from 25 clones were partially or completely sequenced, but only one was found to contain sequences homologous to the soybean SAUR genes. This insert was used as a probe to isolate genomic and cDNA clones of the corresponding gene, which was designated SAUR-ACI
  • The isolation and characterization of the SAUR-AC1 gene of Arabidopsis described in this report serves two important purposes
  • Because Arabidopsis is rather distantly related to the legumes from which the other reported SAUR genes derive, the structural features conserved in SAUR-AC1 should suggest which characteristics are of importance to SAUR genes in general
  • The auxl-7 mutant induces about 45% less SAURAC1 mRNA than the wild type
  • The identification of an auxinregulated SAUR gene of Arabidopsis provides a means to exploit the unique genetic resources of this model system so that the nature and mechanisms of auxin responses can be investigated at the molecular level
  • Work done with soybean ?mal1guxin uq $NAS genes has demonstrated that the redistribution of SAUR expression can be visualized using tissue printing or promoter fusion studies.Similar studies with the SAURAC1 gene may be designed to reveal more subtle defects among the mutants affecting the gravity response

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