Auxin Regulation of the Gibberellin Pathway in Pea

Internode segments were cultured in liquid Murashige and Skoog solution first by cutting 20-mm segments from the uppermost section of 10% to 50% expanded internode 7-8 tissue

Damian P. O'Neill; John J. Ross

2002

Scholarcy highlights

  • Internode segments were cultured in liquid Murashige and Skoog solution first by cutting 20-mm segments from the uppermost section of 10% to 50% expanded internode 7-8 tissue
  • Eight of these segments were placed in a separate petri dish with 10 mL of sterile Murashige and Skoog solution containing indole-3-acetic acid and/or CHX added in 100 ␮L of methanol, or 100 ␮L of methanol in the case of controls
  • Petri dishes were incubated in a controlled environment room with a temperature of 20°C Ϯ 2°C and a photoperiod of 16 h of white light supplied by white fluorescent tubes
  • Incubation times ranged from 4 to 24 h, as detailed for each experiment. This technique was based on the work of Smith
  • Material for northern analysis was immersed in liquid nitrogen and stored at Ϫ70°C till RNA extraction took place
  • PsGA3ox1 does not appear to be a primary auxin response gene, but rather a secondary or “late” gene that responds in turn to the initial auxin up-regulation of a primary gene or genes

Need more features? Save interactive summary cards to your Scholarcy Library.