Platanetin: A Potent Natural Uncoupler and Inhibitor of the Exogenous NADH Dehydrogenase in Intact Plant Mitochondria

An addition of 1 mg/ml BSA at this stage completely reversed the uncoupling effect, as we have shown previously with pentachlorophenol

Patrick Ravanel; Michel Tissut; Roland Douce

2008

Scholarcy highlights

  • All flavonoids which inhibit the electron transfer act in the flavoprotein region of the respiratory chain
  • This activity is probably due to the flavonoid structure and the high lipophilicity of platanetin associated with the presence of the isoprenyl chain
  • Addition of 10 to 200 gM platanetin to the incubation medium triggered cyanide-insensitive 02 consumption. The intensity of this pathway was dependent on the concentration of platanetin and represented respectively 0, 10, and 25 nmol 02-min'-mg-' protein when using 1, 10, and 100 gM platanetin with NADH as substrate
  • Our results show that platanetin is the most potent natural uncoupler known at the present time
  • In the flavone-flavonol series, the comparison between the results obtained with platanetin and other flavonoids was especially interesting
  • The non hydroxylated flavone does not behave as an uncoupler, in contrast with monohydroxylated flavonoids such as the 7-OH flavone, the uncoupling activity of which is comparable to that of isoscutellarein
  • TISSUT M, P RAVANEL, D MACHEREL 1984 Study ofsome natural and synthetic compounds inhibiting the electron transfer in plant mitochondria

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