Cortical microtubule remodelling during strigolactone- and light-mediated growth inhibition of Arabidopsis hypocotyls

This study shows effects of exogenously applied synthetic SL GR24 and inhibitor of SL biosynthesis TIS108 on the organization and dynamics of cortical MT in epidermal cells of light-exposed and etiolated hypocotyls of wild type plants and SL-insensitive Arabidopsis mutant max2-1

Krasylenko

2020

Scholarcy highlights

  • Strigolactones, the carotenoid-derived plant hormones and rhizosphere signaling molecules, were discovered in exogenous allelochemical responses as germination stimulants of Orobanchaceae root parasitic weed
  • This study shows effects of exogenously applied synthetic SL GR24 and inhibitor of SL biosynthesis TIS108 on the organization and dynamics of cortical MT in epidermal cells of light-exposed and etiolated hypocotyls of wild type plants and SL-insensitive Arabidopsis mutant max
  • Under the light/dark regime, GR24 used at two different concentrations, stalled hypocotyl elongation and induced mild radial swelling as compared to mock-treated Col-0
  • We examined the extent of its effect on the percentage of hypocotyl reduction of either light- or dark-grown Col-0 or max mutant seedlings by comparison to the mock treatment
  • Hypocotyl length of etiolated max seedlings after the treatment with 3 μM GR24 was 97.38%±6.92% of mocktreated seedlings, of those treated with 25 μM GR24 – 63.18%±6.39%, and of those treated with μM TIS108 – 19.74%±3.19%, respectively. These results revealed the extent of GR24 and TIS108 effects on hypocotyl elongation, showing that etiolated Col-0 seedlings are less responsive to GR24 as compared to light-grown ones, while at the same time they were more sensitive to TIS108 treatment
  • Grafting experiments showed that SL are transported from roots to shoot in the xylem of Arabidopsis and tomato, which provided insight into SL signalling regulation via localization and transport
  • Microtubule dynamics of etiolated max hypocotyl epidermal cells expressing the GFP-MBD marker treated with 3 μM TIS108

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