Single cell RNA sequencing reveals differential cell cycle activity in key cell populations during nephrogenesis

We find that cell cycle activity distinguishes between the “primed” and “self-renewing” sub-populations of nephron progenitors, with increased levels of the cell cycle related genes Birc5, It is made available under aCC-BY-NC-ND 4.0 International license

Bais

2020

Scholarcy highlights

  • The mammalian kidney has evolved to provide critical adaptive regulatory mechanisms, such as the excretion of waste, and the maintenance of water, electrolyte and acidbase homeostasis to the body
  • We find that cell cycle activity distinguishes between the “primed” and “self-renewing” sub-populations of nephron progenitors, with increased levels of the cell cycle related genes Birc5, It is made available under aCC-BY-NC-ND 4.0 International license
  • Over 30 terminally differentiated nephron cell types are required for the function of the mammalian kidney
  • The advent of scRNA-seq technology has made it possible to explore the cellular heterogeneity of the kidney and precisely identify the transcriptional signatures that define each of its cell types
  • We find similar enrichment for Sine Oculis Homeobox Homolog 2 and Crym in cortical stroma, whereas Gdnf is more modestly enriched in cortical stroma
  • We find that cell cycle activity distinguishes between “primed”
  • Following the same procedure as before we derived a low-dimensional representation and identified six clusters of cells, corresponding to two types of nephron progenitor cells, “mixed/differentiating” cells as well as distal tubular cells and proximal tubular cells

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