Hydroxyl carlactone derivatives are predominant strigolactones in Arabidopsis

In Arabidopsis thaliana, we have demonstrated that MAX1, a cytochrome P450 monooxygenase, converts carlactone into carlactonoic acid, and that LATERAL BRANCHING OXIDOREDUCTASE, a 2-oxoglutarate-dependent dioxygenase, converts methyl carlactonoate into a metabolite called Da



Scholarcy highlights

  • Strigolactones were originally identified as germination stimulants for root parasitic plants and as hyphal branching factors for symbiotic arbuscular mycorrhizal fungi
  • LC-MS/MS analysis of SLs revealed that CL and methyl carlactonoate accumulate in root tissues of lbo mutants
  • We showed that the LATERAL BRANCHING OXIDOREDUCTASE enzyme expressed in E. coli only consumed MeCLA when fed with CL, carlactonoic acid, or MeCLA and converted MeCLA into a product of
  • All were detected in Arabidopsis plants, but CLAs were not detected in max1 mutants
  • The present study demonstrated that recombinant LBO-2 protein is very weak at converting MeCLA into 1'-HO-MeCLA
  • We have progressed with potential enzymatic functions of LBO and MAX1 and their substrates and products downstream of CL in the SL biosynthetic pathway in Arabidopsis
  • As most seed plant species sequenced so far contain a single LATERAL BRANCHING OXIDOREDUCTASE gene, and the LBO gene lineage appears to have been derived deep in plant evolutionary history, the biological function of LBO is likely to be highly conserved in the plant kingdom

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