Glycolipids of Recent Clinical Isolates of Mycobacterium tuberculosis: Chemical Characterization and Immunoreactivity

We have recently identified in M. tuberculosis a major phenolic glycolipid with a unique trisaccharide moiety and related monoglycosyl derivatives

M. Daffe

2009

Scholarcy highlights

  • Tuberculosis is still a serious public health problem today: as many as 8 million new cases occur in the world each year.As glycolipidsfrom mycobacteria are antigenic, and often species-specific, a knowledge of the structure of Mycobacterium tuberculosis specific glycolipid antigens should be useful in developing serodiagnostic methods for tuberculosis
  • In all crude lipid extracts of the ten recent clinical isolates tested, the major glycolipid detected by thin-layer chromatography had a mobility intermediate between those of dimycoloyl trehalose and monomycoloyl trehalose
  • When a triglycosyl phenolic glycolipid was characterized in the Canetti and similar strains of M . tuberculosisit was anticipated and confirmed that it was a useful antigen both for the identification of clinical isolatesand the immunodiagnosis of tuberculosis.The occurrence of the phenolic glycolipid antigen in wild strains of tubercle bacilli was detected using serologicalmethods and its chemical structure was partially established
  • It was suggested that a wide survey of M. tuberculosis strains and clinical isolates could lead to the definition of neotypes
  • The present study showed that the phenolic glycolipid partially characterized by thin-layer chromatography from wild strains of tubercle bacilli had exactly the same chemical structure as the triglycosyl phenolic glycolipid described in the Canetti strains
  • The data both from thin-layer chromatography and from immunogenicity studies in the present work indicated without ambiguity that the glycolipid fractions found in Canetti strain6) were found in wild strains of tubercle bacilli

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