Fatty Acid and Polar Lipid Composition of the Genus Amycolatopsis: Application of Fast Atom Bombardment-Mass Spectrometry to Structure Analysis of Underivatized Phospholipids

' Institut fur Medizinische Mikrobiologie und Immunologie der Universitat Bonn, Sigmund-Freud-Strasse 25, 0-5300Bonn 1, and Institut fur Organische Chemie der Universitat zu Koln, 0-5000Koln 41, Gemzany

A. F. Yassin

2009

Scholarcy highlights

  • ' Institut fur Medizinische Mikrobiologie und Immunologie der Universitat Bonn, Sigmund-Freud-Strasse 25, 0-5300Bonn 1, and Institut fur Organische Chemie der Universitat zu Koln, 0-5000Koln 41, Gemzany
  • Four ninhydrin-positive spots were detected on thin-layer chromatographic plates of the strains studied: two spots cochromatographed with authentic PME and PE, and two more polar spots corresponded to PME and PE acylated with hydroxy fatty acids
  • The co-occurrence of acidic phospholipids with neutral ones is a phenomenon found in many bacteria (17, 23, This characteristic polar lipid pattern readily distinguishes AmycoZatopsis spp. from Nocardia spp. and other mycolic acid-containing taxa that contain DPG and characteristic PI mannosides, as well as from other mycolateless actinomycetes, such as Streptomyces spp. and related wall chemotype I actinomycetes which possess DPG, PE, PI, and PI mannosides
  • Amycolatopsis strains from Actinomadura strains which contain DPG, PI, and monoacylated PI mannosides as major phospholipids
  • The presence of two forms of both PE and PME, one acylated with hydroxylated fatty acids and the other acylated with nonhydroxylated fatty acids, is to be regarded as a new chemotaxonomic characteristic for the differentiation of Amycolatopsis spp. from other actinomycetes
  • The increasing number of reports on the detection of phospholipids acylated with hydroxylated fatty acids in members of the orderActinomycetales should be regarded as an important chemical marker that could be used to differentiate members of this order
  • Collins et al reported the presence of DPG, PG, and two unidentified lipids in strains of Arthrobacter simpla and concluded that the latter might correspond to the hydroxy fatty acid-containing PG found by Yano et al

Need more features? Save interactive summary cards to your Scholarcy Library.