OVER EXPRESSION OF HYPOXIA-INDUCIBLE FACTOR-1α IN RENAL AND BLADDER CANCER CELLS INCREASES TUMORIGENIC POTENCY

We evaluated the effects of Hypoxia-inducible factor-1α over expression on the tumorigenic potency of renal cell carcinoma VMRC cells and bladder cancer EJ cells in vitro and in vivo

YUKIHIRO KONDO; JUNKO HAMADA; CHIE KOBAYASHI; RYOSUKE NAKAMURA; YASUTOMO SUZUKI; RYOJI KIMATA; TAIJI NISHIMURA; TAKAYUKI KITAGAWA; MANABU KUNIMOTO; NOBUMASA IMURA; SHUNTARO HARA

2005

Scholarcy highlights

  • We evaluated the effects of Hypoxia-inducible factor-1α over expression on the tumorigenic potency of renal cell carcinoma VMRC cells and bladder cancer EJ cells in vitro and in vivo
  • Materials and Methods: We introduced HIF-1α expression vectors into VMRC and EJ cells, and generated the HIF-1α over expressing cell lines VMRC-HIF1α and EJ-HIF1α, and the vector only transfected cell lines VMRC-neo and EJ-neo
  • In vitro studies showed that HIF-1α over expression in VMRC and EJ cells accelerated cell proliferation during the confluent growth phase and rendered these cells resistant to hypoxic stress
  • HIF-1α over expression did not affect the size of EJ cell derived xenografts, histological examination showed that there was only a small area of necrosis in EJ-HIF1α cell derived xenografts, whereas a large area of central necrosis was observed in EJ-neo cell derived xenografts
  • It was found that HIF-1α over expression increased intratumor microvessel density in the xenografts. These results suggest that HIF-1α may have important roles in bladder and renal cancer angiogenesis and proliferation
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