Aerobic glycolysis by proliferating cells: a protective strategy against reactive oxygen species 1

We show that: 1) Resting thymocytes meet their ATP demand mainly by oxidative glucose breakdown In contrast to nonstimulated resting thymocytes, production of PMA primed reactive oxygen species Consistent with this finding, no ROS formation is observed in proliferating human pro-myelocytic HL-60 cells, whereas differentiated, nonproliferating HL-60 cells exert a marked response upon priming with PMA. 4) The observed reduction of ROS formation by resting thymocytes incubated with pyruvate suggests a function of pyruvate as an H2O2 scavenger. 5) The respiratory chain is a potential origin for ROS because inhibitors of the mitochondrial electron transport strongly reduce H2O2 production by resting thymocytes

Karl A. Brand; Ulrich Hermfisse

2018

Scholarcy highlights

  • Our laboratory has reported that glucose is essential for glycolytic enzyme induction and proliferation of mitogen-activated rat thymocytes
  • 3) Consistent with this finding, no ROS formation is observed in proliferating human pro-myelocytic HL-60 cells, whereas differentiated, nonproliferating HL-60 cells exert a marked response upon priming with PMA. 4) The observed reduction of ROS formation by resting thymocytes incubated with pyruvate suggests a function of pyruvate as an H2O2 scavenger
  • 5) The respiratory chain is a potential origin for ROS because inhibitors of the mitochondrial electron transport strongly reduce H2O2 production by resting thymocytes
  • We show that: 1) Resting thymocytes meet their ATP demand mainly by oxidative glucose breakdown In contrast to nonstimulated resting thymocytes, production of PMA primed reactive oxygen species Consistent with this finding, no ROS formation is observed in proliferating human pro-myelocytic HL-60 cells, whereas differentiated, nonproliferating HL-60 cells exert a marked response upon priming with PMA. 4) The observed reduction of ROS formation by resting thymocytes incubated with pyruvate suggests a function of pyruvate as an H2O2 scavenger. 5) The respiratory chain is a potential origin for ROS because inhibitors of the mitochondrial electron transport strongly reduce H2O2 production by resting thymocytes
  • The results are discussed in the context of aerobic glycolysis by proliferating cells being a means to minimize oxidative stress during the phases of the cell cycle where maximally enhanced biosynthesis and cell division do occur.—Brand, K
  • Aerobic glycolysis by proliferating cells: a protective strategy against reactive oxygen species

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