pFiD188, the Linear Virulence Plasmid of Rhodococcus fascians D188

Manual curation, and similarity searches, we identified and annotated 184 open reading frames: 91 gene products had a functional homolog, 56 were conserved hypothetical proteins, and 37 were unique for R. fascians

Isolde Francis; Annick De Keyser; Philippe De Backer; Carmen Simón-Mateo; Jutta Kalkus; Ine Pertry; Wilson Ardiles-Diaz; Riet De Rycke; Olivier M. Vandeputte; Mondher El Jaziri; Marcelle Holsters; Danny Vereecke

2012

Scholarcy highlights

  • The sequence data for the linear plasmid pFiD188 of R. fascians D188 have been submitted to the GenBank database under accession number JN093097
  • Rhodococcus jostii RHA1, one of the most effective polychlorinated biphenyl degraders, has three linear plasmids, whereas the genes involved in alkane degradation of R. erythropolis PR4 and those required for isopropylbenzene and chlorocatechol catabolism of R. erythropolis BD2 and R. opacus B4, respectively, are all located on linear plasmids
  • R. equi, the structural organization of pFiD188 of R. fascians is largely similar to that found in the linear plasmids pBD2, pREL1, pRHL2, and pROB01 of R. erythropolis strains BD2 and PR4, R. jostii RHA1, and R. opacus B4, respectively, strongly implying a common origin
  • Just like the related environmental rhodococcal linear plasmids, pFiD188 has a composite structure with conserved regions involved in plasmid maintenance and unique regions implicated in the pathogenic and plant-associated life style of R. fascians
  • In R. fascians, the evolution toward interaction with a plant host and disease elicitation might have occurred through a mechanism that reoriented the function of core actinobacterial traits, combined with acquisition of genuine virulence factors and genes involved in adaptation to the plant niche, possibly via horizontal gene transfer
  • These different classes of gene origin are illustrated by the stk, fas, and nrp loci
  • Vandeputte, personal communication) offers new opportunities and challenges to unravel the pathogenicity strategies of R. fascians

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