Structural dynamics of cereal mitochondrial genomes as revealed by complete nucleotide sequencing of the wheat mitochondrial genome

We demonstrate by gene map comparison that thorough gene shuffling occurred during the speciation of three cereals, leading to remarkable changes in their mitochondrial genome structures, as previously shown by the restriction fragment mapping of maize mitochondrial DNA and by MultiPipMaker analysis of several sequenced plant mitochondrial genomes

Y. Ogihara

2005

Scholarcy highlights

  • The mitochondrial genome is important in plant development, as well as in productivity, and extensive studies have been done on its functions
  • We demonstrate by gene map comparison that thorough gene shuffling occurred during the speciation of three cereals, leading to remarkable changes in their mitochondrial genome structures, as previously shown by the restriction fragment mapping of maize mitochondrial DNA and by MultiPipMaker analysis of several sequenced plant mitochondrial genomes
  • We propose a new method for quantifying genome-wide molecular changes in mitochondrial genomes, which result in ontogenetic as well as phylogenetic variability of the cereal mitochondrial genomes
  • We used a new gene-based strategy for wheat, facilitated by the fact that many wheat mitochondrial genes are available as probes for selecting wheat mitochondrial DNA clones for sequencing. Use of this strategy gave a complete picture of the wheat mitochondrial genome by sequencing the 872.5 kb mtDNA, less than twice the genome size, 452 528 bp
  • Quantification of the subgenomic molecules over the non-recombinants is achieved in the same way as described above. Such studies targeted to different repeat pairs enumerated in Table 2, using Cosmid clones of wheat mtDNA extracted from different organs or different ages of the plant as the template, will disclose structural dynamics of the mitochondrial genome in plant development
  • If the genome consisted of two subgenomic molecules, recombination between the repeat sequences in two separated molecules should produce the master circle molecule, or its double-flop configuration

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