Characterization of growth and development of sorghum genotypes with differential susceptibility to Striga hermonthica

We demonstrate additional differences between Shanqui Red and SRN39 by phenotypic and molecular characterization

Dorota Kawa; Tamera Taylor; Benjamin Thiombiano; Zayan Musa; Hannah E Vahldick; Aimee Walmsley; Alexander Bucksch; Harro Bouwmeester; Siobhan M Brady


Scholarcy highlights

  • Sorghum bicolor is one of the five most important cereal crops globally
  • Shanqui Red and SRN39 are sorghum varieties commonly used as Striga-susceptible and -resistant models, respectively. Their potential differences in whole plant strigolactone composition as well as extensive genetic divergence suggest that additional differences in SRN39 growth and development should be characterized and considered in the interpretation of experiments using SRN39 and SQR as controls
  • To gain insight into biological processes that may differ between these genotypes, we profiled the transcriptomes of 28and 35-day-old plants, the approximate age used for sorghum strigolactone profiling.As anticipated, given the extensive genotypic differences between these varieties, multidimensional scaling revealed a clear separation of transcriptional landscapes between the two genotypes, as well as an effect of plant age
  • Sorghum genotypes producing orobanchol have increased lateral root growth To verify whether the observed increase in root system size of SRN39 could be associated with the LOW GERMINATION STIMULANT 1 polymorphisms, or other genetic differences between SQR and SRN39, we evaluated the root system architecture of three other sorghum varieties: Birhan, Framida, and Gobiye
  • Shanqui Red is a Kaoliang-type sorghum variety of the bicolor race originating from China, while SRN39 is a released Sudanese variety of the caudatum race
  • Gradients of eluent A and eluent B were as follows: 0–1 min; 1–15 min; 15–18 min
  • SQR and SRN39 have been studied mostly in the context of their distinct levels of sensitivity to cold and Striga infection. Despite their vast genetic diversity, the differences between these two genotypes have previously been attributed to a polymorphism in the LGS1 locus leading to changes in composition of their root exudates
  • Expression of strigolactone biosynthesis and signaling pathway genes in roots of 28- and 35-day-old plants of Shanqui Red and SRN39

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