Jasmonate inhibits adventitious root initiation through repression of CKX1 and activation of RAP2.6L transcription factor in Arabidopsis

We found that CK free base content declined during Adventitious root initiation, due to down-regulation of de novo CK biosynthesis and up-regulation of CK inactivation pathways

Asma Dob; Abdellah Lakehal; Ondrej Novak; Catherine Bellini

2021

Scholarcy highlights

  • Unlike most animals,plants have evolved a remarkable capacity to regenerate new organs post-embryonically
  • Kav.The triple mutant ninja-1myc2-322B-35S:CYTOKININ OXIDASE/DEHYDROGENASE1 was generated by crossing ninja-1myc2322B and 35S:RAP2.6L plants.The A. thaliana ecotype Col-0 was used as the wild type and background for all the mutants and the 35S:CKX1 transgenic line, except the 35S:RAP2.6L transgenic line, which was in the Ws-4 background
  • This analysis detected 3778 differentially expressed genes, 2480 of which were up-regulated and 1298 down-regulated at T9 compared with T0
  • These data clearly show that shifting such seedlings from dark to light causes profound transcriptional reprogramming in the hypocotyls, which probably involves changes in the expression of genes that regulate the cell fate decision programs leading to Adventitious root initiation
  • Environmental factors associated with the capacity to develop adventitious roots include light and wounding, and the key phytohormones include CKs, which participate in the control of a plethora of developmental processes
  • Previous studies have firmly established that CKs play a key role in ARI, but provided little knowledge of the mechanisms modulating their pools during early stages of the process
  • The gene expression data presented here suggest that MYC2-mediated JA signaling represses Adventitious root initiation through transcriptional repression of CYTOKININ OXIDASE/DEHYDROGENASE1, which catalyzes irreversible cleavage of CKs

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