Molecular Analyses Reveal Inflammatory Mediators in the Solid Component and Cyst Fluid of Human Adamantinomatous Craniopharyngioma

Given the potential for rapid clinical translation, we further investigated these molecules by examination of gene expression levels in the solid tumor component of adamantinomatous craniopharyngioma

Andrew M Donson

2018

Scholarcy highlights

  • Adamantinomatous craniopharyngioma is an uncommon brain neoplasm accounting for ∼4% of pediatric brain tumors
  • These data indicate that adamantinomatous craniopharyngioma cyst fluids and solid tumor components are characterized by an inflammatory cytokine and chemokine expression pattern
  • ACP Cyst Fluid Is Characterized by High Levels of Cytokines and Chemokines
  • Cyst formation is associated with multiple pediatric brain tumors, most commonly ACPs and pilocytic astrocytoma, and occasionally EPNs, Gangliogliomas and high-grade neoplasms, among others
  • This work provides novel insights regarding the activity of inflammatory mediators in pediatric ACP cyst fluids and corresponding tumor tissue
  • The day after washing the primaries with PBS, we incubated the slides with Goat antirabbit biotinylated antibody at 1:250 dilution for 1 hour at room temperature
  • Through analysis on the transcript and protein levels, we describe elevated levels of IL-6, CXCL1, IL-8, IL-10 and their receptors in ACPs, relative to other pediatric brain tumors and normal cerebral tissue
  • These findings, taken within the context of recent advances in adamantinomatous craniopharyngioma animal models and successful directed therapies in humans, provide a robust platform for multiple preclinical studies, with the potential for rapid translation towards novel directed therapies against pediatric ACPs

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