The winged-helix transcription factor Trident is expressed in cycling cells

We describe the cloning and characterization of Trident, a novel member of the fork head/winged-helix family, from murine thymus

W. Korver; J. Roose; H. Clevers

2002

Scholarcy highlights

  • The family of fork head or ‘winged-helix’ proteins comprises a large number of transcription factors, defined by a conserved DNA binding domain
  • When cells are entering the cell cycle, Trident is expressed upon entry into S phase and phosphorylated during M phase
  • It was shown that the expression is induced in S phase, implicating a role for the protein in the progression of cells through the cell cycle
  • Another observation that links Trident to the cell cycle was made by Westendorf et al, who cloned MPP2, the 3′ end of the human TRIDENT cDNA, in a search for M phase phosphoproteins
  • MPM-2, a monoclonal antibody that binds an epitope containing a phospho amino acid was used to screen bacterially expressed proteins after phosphorylation with extracts from M phase enriched HeLa cells. These results suggest that Trident is phosphorylated upon entry into M phase, presumably by p34cdc2
  • Based on its expression pattern and its phosphorylation in M phase, the Trident protein is likely involved in cell cycle-specific gene regulation

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