Quantitation of Plasma Human Immunodeficiency Virus Type 1 RNA by Competitive Polymerase Chain Reaction

Clinical measures of human immunodeficiency virus type 1 activity in.vivo are limited and hinder the assessment of antiretroviral therapies

D. T. Scadden; Z. Wang; J. E. Groopman

2011

Scholarcy highlights

  • Clinical measures of human immunodeficiency virus type 1 activity in.vivo are limited and hinder the assessment of antiretroviral therapies
  • Reported here is a method for quantitating HIV-I RNA in human plasma using the polymerase chain reaction. This method uses an internal cRNA standard generated from a cloned 113-bp deletion mutation of a highly conserved HIV-I gag region sequence
  • Known quantities ofK4 cRNA added to wild-type HIV-I in a competetive PCR strategy using a radiolabeled primer permitted quantitation of wild-type HIV1 RNA over four orders of magnitude
  • RNA isolated from plasma from AIDS patients yielded 103 to 8 × 104 HIV-I RNA copies/ml of plasma with an average intrasampie coefficient of variation of
  • This method offers a sensitive assay with a broad dynamic range for monitoring HIV-I activity in the plasma of AIDS patients
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