Clinical Evaluation of Branched DNA Signal Amplification for Quantifying HIV Type 1 in Human Plasma

Quantification of HIV-1 RNA in human plasma has provided unique insight into AIDS pathogenesis and promises to hasten progress in antiretroviral therapy and vaccine research

YUNZHEN CAO; DAVID D. HO; JOHN TODD; ROBERT KOKKA; MICKEY URDEA; JEFFREY D. LIFSON; MICHAEL PIATAK; SHANDE CHEN; BEATRICE H. HAHN; MICHAEL S. SAAG; GEORGE M. SHAW

2009

Key concepts

Scholarcy highlights

  • Quantification of HIV-1 RNA in human plasma has provided unique insight into AIDS pathogenesis and promises to hasten progress in antiretroviral therapy and vaccine research
  • No generally available HIV-1 RNA assay has yet been subjected to rigorous clinical testing or to comparative evaluation with research-based RNA assays using large numbers of well-characterized clinical specimens
  • Plasma viral RNA determinations by branched DNA and quantitative competitive polymerase chain reaction assays were quantitatively similar in the range of 104 to 107 RNA molecules/ml and declined identically following the institution of zidovudine therapy
  • The close quantitative correlation between branched DNA and quantitative competitive polymerase chain reaction results, and their significant association with other viral markers and CD4+ counts, support the use of plasma viral RNA measurement in HIV-1 clinical trials

Need more features? Save interactive summary cards to your Scholarcy Library.