Acetylcholine Receptors

Using 26I-labeled a-bungarotoxin and autoradiography, Hartzell and Fambrough reported upon the distribution of acetylcholine receptors in denervated rat diaphragm, the number of binding sites per unit area of surface membrane, and the relation between these values and corresponding acetylcholine sensitivities determined by iontophoresis

Douglas M. Fambrough

2004

Scholarcy highlights

  • Using 26I-labeled a-bungarotoxin and autoradiography, Hartzell and Fambrough reported upon the distribution of acetylcholine receptors in denervated rat diaphragm, the number of binding sites per unit area of surface membrane, and the relation between these values and corresponding acetylcholine sensitivities determined by iontophoresis
  • The best possible values of acetylcholine receptor density in denervated muscle membranes are needed for comparison with data from freeze-fracture studies of these membranes, for a quantitative picture of receptor function in relation to acetylcholine "noise" studies and as a basis for interpreting chemosensitivity measurements
  • Isolation and lodination of a-Bungarotoxin a-Bungarotoxin was isolated from the venom of Bungarus multicinctus, obtained from the Miami Serpentarium, and was purified and characterized as previously described
  • Rat diaphragm undergoes a transient hypertrophy after denervation
  • Muscle fiber circumferences and a-bungarotoxin binding site densities were determined as described in Methods
  • Rash for advice on the fixation and processing of muscle to obtain fiber circumference measurements

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