Elevated neuronal nitric oxide synthase expression during ageing and mitochondrial energy production

This study evaluated the effect of ageing on brain mitochondrial function mediated through protein post-translational modifications

Philip Y. Lam

2009

Scholarcy highlights

  • Nitric oxide, a free radical formed in a variety of cell types by nitric oxide synthases, is involved in a wide array of physiological and pathophysiological phenomena
  • The present study reports on increased cytosolic Neuronal nitric oxide synthase expression with age and, as mentioned above, the NO effects on mitochondria are a function of cytosol-generated NO diffusing to these organelles
  • Rat brain nNOS protein level is elevated during ageing
  • The total nNOS content in rat brain homogenate increased during ageing, as detected by Western blot using an antibody targeting the C-terminal of nNOS
  • A specific nNOS band with a molecular weight ~155 KDa, which corresponds to the cytosolic form of nNOS, was observed
  • The existence of mitochondrial nitric oxide synthase has been reported in experiments using Wistar rats, suggesting that the expression of mtNOS is strain-specific
  • The cytochrome c release observed as a function of age correlated with the increase in cytosolic Neuronal nitric oxide synthase activity and may be ascribed to excessive formation of ONOO− in mitochondria

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