Proteome Variations in Pancreatic Stellate Cells upon Stimulation with Proinflammatory Factors

This study aims to define molecular events at the level of the proteome that occur as a consequence of pancreatic stellate cells activation with individual proinflammatory mediators

Aseel J. Marzoq; Nathalia Giese; Jörg D. Hoheisel; Mohamed Saiel Saeed Alhamdani

2013

Scholarcy highlights

  • Stroma formation in pancreatic cancer is controlled via the activation of pancreatic stellate cells
  • This study was aimed at investigating molecular changes at the level of the proteome that are associated with the activation of pancreatic stellate cells by proinflammatory factors, namely TNF-␣, FGF2, IL6, and chemokine ligand 4
  • CCL4 is associated with cellular neovascularization, whereas FGF2 and IL6 induction led to better cellular survival and decreased apoptotic activity of the stellate cells
  • pancreatic stellate cells play a central role in pancreatic fibrogenesis during inflammation and cancer
  • Several mediators are involved in the communication process between inflammatory cells, tumor cells, and PSCs
  • We found that cell growth with 10% serum caused a significant change in the expression level of 230 proteins. 47 of them were not observed with the four cytokines alone and have unique functional characteristics
  • This study evaluates the contribution of TNF-␣, FGF2, IL6, and CCL4 to the activation and proliferation
  • An overview of the influence and combinatorial effect of the four pro-inflammatory factors on pancreatic stellate cells can be generated that is on the basis of an analysis at the protein level and the functional characteristics that are associated to them

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